Article 8418

Title of the article

TESTING A NEW NUCLEAR DNA MARKER FOR STUDIES OF HYBRIDIZATION IN SPECKLED (SPERMOPHILUS
SUSLICUS GÜLD.) AND LARGE GROUND SQUIRRELS 

Authors

Titov Sergey Vital'evich, Doctor of biological sciences, professor, head of sub-department of zoology and ecology, dean of the Faculty of Physics, Mathematics and Natural Sciences, Penza State University (40 Krasnaya street, Penza, Russia),
E-mail: svtitov@yandex.ru
Kuz'min Anton Alekseevich, Candidate of biological sciences, associate professor, sub-department of biotechnology and technosphere safety, Penza State Technological University (1a/11 Baidukova lane/Gagarina street, Penza, Russia),
E-mail: kuzmin-puh@yandex.com
Zaks Svetlana Sergeevna, Candidate of biological sciences, senior researcher, research and educational laboratory of molecular ecology and systematics of animals, Penza State University (40 Krasnaya street, Penza, Russia), E-mail: s.s.zaks@yandex.ru
Chernyshova Ol'ga Valer'evna, Postgraduate student, Penza State University (40 Krasnaya street, Penza, Russia),
E-mail: oliarabbit@yandex.ru 

Index UDK

591.557:599.322.2 

DOI

10.21685/2307-9150-2018-4-8 

Abstract

Background. Researching hybrid populations in space and time is an urgent task of evolutionary biology and population ecology. Despite a rather successful study of modern hybrid zones of ground squirrels (Rodentia, Sciuridae, Spermophilus), there are questions about the use of molecular genetic markers in the process of observation of hybrid populations. The aim of the study was to study the effectiveness of the previously unused marker of the nuclear DNA fragment of the HOX B5 gene in the diagnosis of species belonging to individuals in the contact colonies of large and speckled ground squirrels.
Materials and methods. Genetic material was collected in Ulyanovsk region during the expedition to study the hybrid zone of large and speckled ground squirrels in 2018. An analytical sample included 28 individuals of S. suslicus, 41 individuals S. major and 5 interspecific hybrids identified by other molecular genetic markers. The obtained 69 HOX b5 gene fragments of large and speckled ground squirrels were sequenced. A genetic analysis of the obtained sequence of HOX B5 gene fragment was carried out using MEGA 7.0.21 and DnaSP 5.10 software package. The obtained nucleotide sequences revealed species-specific features of this marker.
Results. The analysis of the nucleotide sequences of a fragment of the gene HOX b5 (716 bp) in large ground squirrels (n = 41) revealed 5 haplotypes, and in speckled ground squirrels (n = 28) – only two haplotype. The analyzed fragment of the nuclear marker HOX b5 contains both sites that reliably differentiate haplotypes in each of the hybridizing species, and a site that has a species-specific replacement G–C (position 308), creating a site for recognition of the restriction endonuclease BmeI 3901.
Conclusions. The results indicate a good diagnostic ability of this molecular genetic marker in the study of the genetic status of individuals from contact colonies on the conducted studies of the variability of nucleotide sequences of the HOX B5 gene in two species of hybridizing ground squirrels and the identification of speciesspecific nucleotide features of these sequences. 

Key words

large ground squirrel, speckled ground squirrel, interspecific hybridization, nuclear DNA, HOX b5 gene, PCR-RFLP analysis of hybrids 

 

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Дата создания: 22.03.2019 10:07
Дата обновления: 22.03.2019 10:56